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Crystalgen Inc. New Product

Usage Instruction for Dyehard Rapid 5-Min Solution

Crystalgenís Dyehard 5-min staining solution is a new improved Coomassie Briliant Blue

Staining of proteins in polyacrylamide gels including isoelectric focusing gels with water clear

background at high sensitivity. The new formula takes advantage of making Coomassie

Brilliant

molecules in a polymer form so that a short staining time and higher sensitivity are obtained as

well as clear background.

Features:

1. Simple of use: Only one step of staining.

2. Fast: only 5 minutes

3. Background: Clear water background

4. Sensitivity: 10ng/band.

Protocol:

1. Place the gel in the clean tray or container post the gel electrophoresis.

2. Add 100ml (if the gel is a mini-gel) of the staining solution to the container with the gel.

3. Cover the container with a lid and place it on the shaker and shake the container at

50-100rpm. Bands will be visible in 5 min.

4. Rinse the gel with deionized water, two changes of water. The gel will have

A clear background and ready for photograph. Note: the gel can be left in water for a few days without

change in band intensity and background clarity.

Order information:

Cat# 300-692-500R Rapid 5-min staining solution, 500ml, ask

Cat# 300-692-1000R Rapid 5-min staining solution, 1L, ask

Crystalgen Inc. New Product

Usage Instruction for Dyehard 2-hour Solution

Crystalgenís Dyehard 2-hour staining solution is a new improved Coomassie Briliant Blue

Staining of proteins in polyacrylamide gels including isoelectric focusing gels with water clear

background at high sensitivity. The new formula takes advantage of making Coomassie

Brilliant molecules in a polymer form so that a short staining time and higher sensitivity are

obtained as well as clear background.

Features:

1. Simple of use: Only one step of staining.

2. Fast: only 2-hour.

3. Background: Clear water background

4. Sensitivity: 3 ng/band.

Protocol:

1. Place the gel in the clean tray or container post the gel electrophoresis.

2. Add 100ml (if the gel is a mini-gel) of the staining solution to the container with the gel.

3. Cover the container with a lid and place it on the shaker and shake the container at

50-100rpm. Bands will be visible in 30-min.

4. Rinse the gel with deionized water, two changes of water. The gel will have

A clear background and ready for photograph. Note: the gel can be left in water for a few days without

change in band intensity and background clarity.

Order information:

Cat# 300-692-500 Rapid 5-min staining solution, 500ml, ask

Cat# 300-692-1000 Rapid 5-min staining solution, 1L, ask

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Last modified: 09/28/09