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LB Agar powder (lennox A agar) 500 g

LB Agar powder (lennox A agar) 2,5 kg

LB Broth (1X), liquid 500 ml

LB Broth Base, powder 500 g

LB Broth Base, powder 2,5 kg

Luria Broth Base, powder 500 g

Luria Broth Base, powder 2,5 kg

S.O.C. Medium, liquid 10 X 10 ml

Select Agar, powder 500 g

Select Agar, powder 2,5 kg

Terrific Broth, powder 500 g

2-YT Broth, powder 500 g

Zeocin 1 g

Zeocin 5 g

Actinomycin D 5 mg

Ampicillin - sodium salt irradiated 200 mg

Cefotaxime, Sodium Salt 2 g

Kanamycin Sulfate, powder 5 g

Kanamycin Sulfate, powder 25 g

Kanamycin Sulfate, liquid 100 ml

Streptomycin Sulfate 100 g

Tetracycline 5 g

Carbenicillin, Disodium Salt 5 g

LOT 2: Cloning, Microbiology and Reagents - ANNEX A

1/5

Lot 2: Cloning, microbiology and reagents - Annex A

X-gal 100 mg

X-gal 1 g

Bluo-gal 1 g

IPTG 1 g

Electrophoresis and nucleic acid purification

Simply Blue SafeStain 1 L

Or equivalent ready-to-use Coomassieģ G-250 stain specifically designed for fast, sensitive, and safe protein

staining. Possibility to detect as little as 7 ng of protein. It shoul be completely non-hazardous / not need of acetic

acid/methanol fixative or destain. Compatible with mass spectrometry.

Simply Blue SafeStain 3,5 L

Or equivalent ready-to-use Coomassieģ G-250 stain specifically designed for fast, sensitive, and safe protein

staining. Possibility to detect as little as 7 ng of protein. It shoul be completely non-hazardous / not need of acetic

acid/methanol fixative or destain. Compatible with mass spectrometry.

SYBR Safe DNA Gel Stain in DMSO 400 ul

Or equivalent DNA gel stain with reduced mutagenicity to be safer than ethidium bromide for staining DNA in

agarose or acrylamide gels. Not only less mutagenic than ethidium bromide, but with stain's detection sensitivity

better than that of ethidium bromide. Stain has to be as a premixed solution that can be used just like an ethidium

bromide solution.

SYBR Safe DNA Gel Stain in 0.5X TBE 1 L

Or equivalent DNA gel stain with reduced mutagenicity to be safer than ethidium bromide for staining DNA in

agarose or acrylamide gels. Not only less mutagenic than ethidium bromide, but with stain's detection sensitivity

better than that of ethidium bromide. Stain has to be as a premixed solution that can be used just like an ethidium

bromide solution.

SYBR Safe DNA Gel Stain in 0.5X TBE 4 L

Or equivalent DNA gel stain with reduced mutagenicity to be safer than ethidium bromide for staining DNA in

agarose or acrylamide gels. Not only less mutagenic than ethidium bromide, but with stain's detection sensitivity

better than that of ethidium bromide. Stain has to be as a premixed solution that can be used just like an ethidium

bromide solution.

SYBR Safe DNA Gel Stain in 1X TAE 1 L

Or equivalent DNA gel stain with reduced mutagenicity to be safer than ethidium bromide for staining DNA in

agarose or acrylamide gels. Not only less mutagenic than ethidium bromide, but with stain's detection sensitivity

better than that of ethidium bromide. Stain has to be as a premixed solution that can be used just like an ethidium

bromide solution.

SYBR Safe DNA Gel Stain in 1X TAE 4 L

Or equivalent DNA gel stain with reduced mutagenicity to be safer than ethidium bromide for staining DNA in

agarose or acrylamide gels. Not only less mutagenic than ethidium bromide, but with stain's detection sensitivity

better than that of ethidium bromide. Stain has to be as a premixed solution that can be used just like an ethidium

bromide solution.

Streptavidin, R-phycoerythrin conjugate 1 ml

RNase out recomb ribonuclease inhibitor 5000 Units

Recombinant Ribonuclease Inhibitor: a potent non-competitive inhibitor of pancreatic-type ribonucleases such as

RNase A, RNase B, and RNase C.

Rnase AWAY 250 ml

a ready-to-use solution for eliminating RNase and DNA contamination from labware

PicoGreen dsDNA Quantitation Assay (2000 assays) 1 kit

Or equivalent quantitation Assay to selectively detect as little as 25 pg/ml of dsDNA in the presence of ssDNA,

RNA, and free nucleotides. Accurately measure DNA from many sources, including genomic DNA, miniprep DNA,

or PCR amplification products. Orders of magnitude more sensitive than UV absorbance readings, saving on

precious sample. Specific for dsDNA in the presence of equimolar amounts of RNA. Easy to use. Suitable for use

in 96- and 384-well plate formats. Compatible with most fluorescence-based microplate readers and fluorometers

and Fluorescence.

FluoroPure Grade Hoechst 33258 100 mg

Nuclear counterstain that emits blue fluorescence when bound to dsDNA .ISO 9001-certified.

Cell Culture

Advanced D-MEM (Dulbecco's Modified Eagle Medium) (1X), liquid 500 ml

with 4500 mg/L D-Glucose, Non Essential Amino Acids, 110 mg/L of sodium pyruvate

without L-Glutamine

Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (High Glucose) 500 ml

with L-Glutamine, 4500 mg/L D-Glucose

without sodium pyruvate

Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (High Glucose) 1000 ml

with L-Glutamine, 4500 mg/L D-Glucose

without sodium pyruvate

Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (High Glucose) 500 ml

with L-Glutamine, 4500 mg/LD-Glucose, Sodium Pyruvate

Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (High Glucose) 500 ml

with L-Glutamine, 4500 mg/L D-Glucose, 25 mM HEPES

2/5

Lot 2: Cloning, microbiology and reagents - Annex A

without sodium pyruvate

Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (High Glucose) 500 ml

with L-Glutamine, 4500 mg/L D-Glucose, 25 mM HEPES

without sodium pyruvate, phenol red

Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (High Glucose) 500 ml

with L-Glutamine, 4500 mg/L D-Glucose, Pyridoxine Hydrochloride

without sodium pyruvate

Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (High Glucose) 5 L

with L-Glutamine, 4500 mg/L D-Glucose

without sodium pyruvate, sodium bicarbonate

Dulbecco's Modified Eagle Medium (D-MEM) (1X), powder (High Glucose) 10 L

with L-Glutamine, 4500 mg/L D-Glucose

without sodium pyruvate, sodium bicarbonate

Dulbecco's Modified Eagle Medium (D-MEM) (1X), powder (High Glucose) 10 L

with L-Glutamine, 4500 mg/L D-Glucose, 110 mg/L Sodium Pyruvate

without sodium bicarbonate

Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (High Glucose) 500 ml

with 4500 mg/L D-Glucose, Non Essential Amino Acids

without L-Glutamine, sodium pyruvate

Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (High Glucose) 500 ml

with 4500 mg/L D-Glucose

without L-Glutamine, sodium pyruvate

Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (High Glucose) 500 ml

with 4500 mg/L D-Glucose, sodium pyruvate

without L-Glutamine

Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (High Glucose) 500 ml

with 4500 mg/L D-Glucose

without L-Glutamine, sodium pyruvate, phenol red

Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (High Glucose) 500 ml

with 4500 mg/L D-Glucose

without L-Glutamine, sodium pyruvate, L-Methionine, L-Cystine

Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (High Glucose) 500 ml

with 4500 mg/L D-Glucose

without L-Glutamine, sodium pyruvate,calcium chloride

Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (High Glucose) 500 ml

with L-Glutamine in a stabilised form, 4500 mg/L D-Glucose, Sodium Pyruvate

Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (High Glucose) 500 ml

with L-Glutamine in a stabilised form, 4500 mg/L D-Glucose, 25 mM HEPES

without Sodium Pyruvate

Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (High Glucose) 500 ml

with L-Glutamine in a stabilised form, 4500 mg/L D-Glucose

without Sodium Pyruvate

Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (Low Glucose) 500 ml

with L-Glutamine, 1000 mg/L D-Glucose, Sodium Pyruvate

Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (Low Glucose) 500 ml

with L-Glutamine

without Glucose, Sodium Pyruvate

Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (Low Glucose) 500 ml

with L-Glutamine, 1000 mg/L D-Glucose, Sodium Pyruvate, 25 mM HEPES

Dulbecco's Modified Eagle Medium (D-MEM) (1X), powder (Low Glucose) 10 L

with L-Glutamine, 1000 mg/L D-Glucose, Sodium Pyruvate

without Sodium Bicarbonate

Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (Low Glucose) 500 ml

with 1000 mg/L D-Glucose, Sodium Pyruvate

without L-Glutamine, Phenol Red

Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (Low Glucose) 500 ml

with L-Glutamine in a stabilised form, 1000 mg/L D-Glucose, Sodium Pyruvate

F-12K 500 ml

with L-Glutamine

Dulbecco's modified Eagle's medium low glucose

without phenol red 500 ml

Minimum Essential Medium (MEM)1x 500 ml

RPMI 1640 Medium (1X), liquid, with L-Glutamine 100 ml

RPMI 1640 Medium (1X), liquid, with L-Glutamine 500 ml

RPMI 1640 Medium (1X), liquid, with L-Glutamine 1000 ml

3/5

Lot 2: Cloning, microbiology and reagents - Annex A

RPMI 1640 Medium (1X), liquid, with L-Glutamine / without Phenol Red 500 ml

RPMI 1640 Medium (1X), liquid, with L-Glutamine, 25 mM Hepes 100 ml

RPMI 1640 Medium (1X), liquid, with L-Glutamine, 25 mM Hepes 500 ml

RPMI 1640 Medium (1X), liquid, with L-Glutamine / without Folic Acid 500 ml

RPMI 1640 Medium, powder, with L-Glutamine / without Sodium Bicarbonate 1 L

RPMI 1640 Medium, powder, with L-Glutamine / without Sodium Bicarbonate 10 L

RPMI 1640 Medium, powder, with L-Glutamine, 25 mM HEPES / without Sodium Bicarbonate 1 L

RPMI 1640 Medium, powder, with L-Glutamine, 25 mM HEPES / without Sodium Bicarbonate 10 L

RPMI 1640 Medium (1X), liquid with L-Glutamine / without Glucose 500 ml

RPMI 1640 Medium (1X), liquid, without L-Glutamine 100 ml

RPMI 1640 Medium (1X), liquid, without L-Glutamine 500 ml

RPMI 1640 Medium (1X), liquid, without L-Glutamine, Phenol Red 500 ml

RPMI 1640 Medium (1X), liquid, with 25 mM Hepes / without L-Glutamine 500 ml

RPMI 1640 Medium (1X), liquid, with 20 mM Hepes, 0.85 g/L Sodium Bicarbonate / without L-Glutamine 500 ml

RPMI 1640 Medium (1X), liquid, with 20 mM Hepes, 1 g/L Sodium Bicarbonate / without L-Glutamine 500 ml

RPMI 1640 Medium (1X), liquid, with L-Glutamine in a stabilised form 500 ml

RPMI 1640 Medium (1X), liquid, with L-Glutamine in a stabilised form, 25 mM HEPES 500 ml

Trypsin (1X), liquid 100 ml

Trypsin (10X), liquid 100 ml

Trypsin-EDTA (1X), liquid 100 ml

Trypsin-EDTA (10X), liquid 100 ml

Penicillin/Streptomycin Solution, liquid 100 ml

Penicillin-Streptomycin-Glutamine (100X), liquid 100 ml

Penicillin-Streptomycin-Neomycin (PSN) Antibiotic Mixture (100X), liquid 100 ml

Streptomycin Sulphate 100 g

HEPES Buffer Solution 1M, liquid 20 ml

HEPES Buffer Solution 1M, liquid 100 ml

Sodium Bicarbonate 7.5%, liquid 100 ml

Trypan Blue Stain, 0.4% 100 ml

MEM Amino Acids Solution (50X), liquid 100 ml

MEM Non Essential Amino Acids (100X) 100 ml

L-Glutamine 200 mM (100X), liquid 20 ml

L-Glutamine 200 mM (100X), liquid 100 ml

Fibronectin, Human Plasma 5 mg

Fungizone Antimycotic, liquid 20 ml

Fungizone Antimycotic, liquid 50 ml

N-2 Supplement, (100X) liquid 5 ml

B-27 Supplement (50X), liquid 10 ml

B-27 Supplement without Antioxidants (50X), liquid 10 ml

B-27 Supplement Minus Vitamin A (50X), liquid 10 ml

G-5 Supplement (100X), liquid 1 ml

Opti-MEM I Reduced serum Medium (1X), liquid 500 ml

with L-Glutamine, 2400 mg/L Sodium Bicarbonate, HEPES, Sodium Pyruvate, Hypoxanthine, Thymidine, Trace

Elements,

Growth Factors, 1.1 mg/L Phenol Red

Opti-MEM I Reduced serum Medium (1X), liquid 500 ml

with L-Glutamine, 2400 mg/L Sodium Bicarbonate, HEPES, Sodium Pyruvate, Hypoxanthine, Thymidine, Trace

Elements,

Growth Factors

Opti-MEM I Reduced serum Medium, powder 10 L

with L-Glutamine, HEPES, Sodium Pyruvate, Hypoxanthine, Thymidine, Trace Elements,

Growth Factors, 1.1 mg/L Phenol Red

Balanced Salt Solutions

Hanks' Balanced Salt Solution (HBSS) (1X), liquid / with Calcium, Magnesium, Phenol Red 100 ml

Hanks' Balanced Salt Solution (HBSS) (1X), liquid / with Calcium, Magnesium, Phenol Red 500 ml

Hanks' Balanced Salt Solution (HBSS) (1X), liquid / without Phenol Red 500 ml

Hanks' Balanced Salt Solution (HBSS) (1X), liquid / without Calcium, Magnesium 100 ml

Hanks' Balanced Salt Solution (HBSS) (1X), liquid / without Calcium, Magnesium 500 ml

Hanks' Balanced Salt Solution (HBSS) (1X), liquid / without Calcium, Magnesium, Phenol Red 100 ml

Hanks' Balanced Salt Solution (HBSS) (1X), liquid / without Calcium, Magnesium, Phenol Red 500 ml

Hanks' Balanced Salt Solution (HBSS) (1X), liquid / without Calcium, Magnesium, Phenol Red, Sodium

Bicarbonate

500 ml

Hanks' Balanced Salt Solution (HBSS) (1X), liquid / without Phenol Red, Sodium Bicarbonate 500 ml

Hanks' Balanced Salt Solution (HBSS) (1X), liquid / without Sodium Bicarbonate 500 ml

Hanks' Balanced Salt Solution (HBSS) (1X), liquid / without Calcium, Magnesium, Sodium Bicarbonate 500 ml

4/5

Lot 2: Cloning, microbiology and reagents - Annex A

Phosphate-Buffered Saline (PBS) (1X), liquid / pH 7.2 500 ml

Phosphate-Buffered Saline (PBS) (10X), liquid / pH 7.2 500 ml

Phosphate-Buffered Saline (PBS) (1X), liquid / pH 7.4 500 ml

Phosphate-Buffered Saline (PBS) (10X), liquid / pH 7.4 500 ml

PBS Tablets 100 tablets

Earle's Balanced Salt Solution (EBSS) (1X), liquid, with calcium, magnesium, phenol red 500 ml

Earle's Balanced Salt Solution (EBSS) (1X), liquid, without calcium, magnesium, phenol red 500 ml

Dulbecco's Phosphate Buffered Saline (D-PBS) (1X), liquid / with calcium, Magnesium 500 ml

Dulbecco's Phosphate Buffered Saline (D-PBS) (10X), liquid / with calcium, Magnesium 500 ml

Dulbecco's Phosphate Buffered Saline (D-PBS) (1X), liquid / without calcium, Magnesium 500 ml

Dulbecco's Phosphate Buffered Saline (D-PBS) (10X), liquid / without calcium, Magnesium 500 ml

Dulbecco's Phosphate Buffered Saline (D-PBS), powder / without calcium, Magnesium 500 ml

Animal Sera

Foetal Bovine Serum Certified / origin: USA / with origin and analysis certificate 100 ml

Foetal Bovine Serum Certified / origin: USA / with origin and analysis certificate 500 ml

Foetal Bovine Serum / origin: Australia / with origin and analysis certificate 100 ml

Foetal Bovine Serum / origin: Australia / with origin and analysis certificate 500 ml

Foetal Bovine Serum / origin: E.U. Approved / with origin and analysis certificate 100 ml

Foetal Bovine Serum / origin: E.U. Approved / with origin and analysis certificate 500 ml

Foetal Bovine Serum Certified (Heat-Inactivated) / origin: USA / with origin and analysis certificate 100 ml

Foetal Bovine Serum Certified (Heat-Inactivated)/ origin: USA / with origin and analysis certificate 500 ml

Foetal Bovine Serum (Heat-Inactivated)/ origin: Australia / with origin and analysis certificate 100 ml

Foetal Bovine Serum (Heat-Inactivated)/ origin: Australia / with origin and analysis certificate 500 ml

Foetal Bovine Serum (Heat-Inactivated)/ origin: E.U. Approved / with origin and analysis certificate 100 ml

Foetal Bovine Serum (Heat-Inactivated)/ origin: E.U. Approved / with origin and analysis certificate 500 ml

Horse Serum / origin: New Zeland / with origin and analysis certificate 100 ml

Horse Serum / origin: New Zeland / with origin and analysis certificate 500 ml

Horse Serum (Heat Inactivated) / origin: New Zeland / with origin and analysis certificate 100 ml

Horse Serum (Heat Inactivated) / origin: New Zeland / with origin and analysis certificate 500 ml

Newboarn Calf Serum (Orig New Zealand) 500 ml

5/5

Lot 2: Cloning, microbiology and reagents - Annex A

Contact Information

Telephone
0032 1658 9045
FAX
0032 1650 9045
Postal address
Voortstraat 49, 1910 Kampenhout, BELGIUM.
Electronic mail
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Last modified: 09/28/09