Product Name: RNAStay RNA Stabilization Solution
1. Product Description
RNA degradation is a serious problem and very hard to prevent since RNA is even
degraded in -80°C or liquid nitrogen storage. RNAStay™ Solution is a revolution
reagent that can stabilize and protect RNA in tissues or cells at room temperature and
thus allows users to isolate the intact RNA or downstream storage of the treated tissue
without the risk of RNA degradation . RNAStay™ is an aqueous, non-toxic tissue and
cell storage reagent that stabilizes and protects cellular RNA in intact, unfrozen tissue and
cell samples. More conveniently, RNAStay™ eliminates the need to immediately process
samples or to freeze samples in liquid nitrogen for later processing. Samples treated with
RNAStay™ can be used with various downstream applications including mRNA and total
RNA isolation kits such as phenol based kit or affinity binding isolation kit. Samples can
be used for histology and immunocytochemistry or continued storage in liquid nitrogen
for a long period without the risk of RNA degradation.
2. Features and Benefits
Rapid Protection Rapidly permeates tissues to stabilize and protect cellular RNA
with immediate RNAse inactivation.
Highly Quality No compromise in RNA quality following mRNA or total RNA
Stable Stabilizes samples at room temperature for up to 1-4 weeks or
Indefinitely at -20°C for archiving needs.
Safe Aqueous non-toxic solution allows downstream tissue processing
a. Small organs, such as rat spleen, kidney, liver can be stored in whole in
RNAStay™ . For large organ or tissues, cut tissue samples to be stored and into less
than 0.5-0.8cm in one dimension.
b. Quickly rinse the samples with RNAStay™ solution.
c. Submerge the samples in 5-6 volumes of RNAStay™ for 5-10 min. When ready to
isolate the RNA, remove the tissue from RNAStay™ and process as though just
harvested. For cell storage, wash pelleted cells in a small amount of 1x PBS and add
5-10 volumes of RNAStay™ Centrifuge at >5000rpm to pellet cells and discard
supernatant before isolate the RNA.
4. Applications of RNAStay™
Genomic DNA from RNAStay™ Solution-Stored samples
DNA can be isolated from RNAStay™ Solution-stored samples.
Isolating protein from RNAStay™ Solution-stored samples.
Proteins are also preserved in RNAStay™ Solution. RNAStay™ Solution will denature
proteins, therefore, protein obtained from samples stored in it will be suitable for
applications such as Western blotting or 2D gel electrophoresis, but not for applications
that require native protein.
5. High Quality of intact RNA
RNA from a colon cancer tissue storage in RNAStay™ solution and
Analysised with Agilent 2100 BioAnalzer to show the integrity
(red line). The data shows that there is no degradation in integrity
6. Yield of RNA
Tissue Mouse ug/mg Tissue Rat Yield ug/mg Tissue
Brain 0.65 0.54
Embryo 2.15 1.90
Heart 0.42 0.43
Kidney 1.50 1.80
Liver 3.40 3.10
Lung 0.49 0.85
Spleen 2.40 2.0
Testicle 1.70 1.40
Thymus 2.05 1.95
Mouse and rat tissues were storage in RNAStay™ solution for 1 hours at 4 °C and then
Processed with the phenol based purification.
7. Guidelines for Use of RNAStay™ Solution
Before immersion in RNAStay™ Solution, cut large tissue samples to 0.5-0.8cm in any
• Use RNAStay™ Solution with fresh tissue only; do not freeze tissue before
immersion in RNAStay™ Solution.
• Place the fresh tissue in 5-10 volumes of RNAStay™ Solution.
• Most samples in RNAStay™ Solution can be stored at room temp for 1-4 weeks
without compromising RNA quality, or at -20°C or -80°C indefinitely.
• Do not freeze samples in RNAStay™ Solution, store at 4°C overnight(to allow
the solution to thoroughly penetrate the tissue), remove supernatant, then move to
-20°C or -80°C for long-term storage.
• Plant tissues may be extended the length of time such as 20-30 minute for fully
reaction and protection.
Storage and Stability
• Store RNAStay™ Solution at room temperature.
• If any precipitation occurs, heat it to 45°C and agitate to re-dissolve it.
Disposal of RNAStay™ Solution
RNAStay™ Solution can be safely discarded down the sink and flushed with water.
Chemical reactivity with oxidants
RNAStay™ Solution is known to react with oxidizing agents, such as bleach. The
reaction releases toxic chlorine gas, and generate heat. If you suspect that samples
may contain bleach, work in a fume hood with adequate protective clothing and
Range of Product applications
RNAStay™ has been proven effective for RNA preservation in animal tissue, E. coli,
Drosophila, tissue culture cells, white blood cells, and most plant tissues.
RNase inactivation is reversible; do not rinse RNAStay™ Solution from samples
before using. Blot tissues with a wipe, or pellet cells to remove excess RNAStay™
Product Cat# Packing Price $
RNAS-50 50ml 49.0
RNAS-250 250ml 179.0
RNAS-500 500ml 290.0
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