he Evaluation of interleukin-4 and interleukin–13 in the Serum of Pulmonary Sarcoidosis and Tuberculosis Patients
by Ronald
No Comments
Background: Sarcoidosis and tuberculosis (TB) are two granulomatous inflammatory disease with some common symptoms. The purpose of this study was to compare the levels of serum biomarkers include interleukin-4 (IL-4) and IL-13, calcium (Ca), hemoglobin, sedimentation rate, and the ratio of lymphocyte-to-neutrophil among patients with pulmonary TB, patients with sarcoidosis and a control group.
Materials and Methods: This case-control study conducted in patients referred to hospital Daneshvari Still, Tehran, from April 2017 up to 2018. In this study, 24 newly diagnosed patients with active pulmonary tuberculosis, 34 patients with pulmonary sarcoidosis, and 30 healthy individuals as the control group were enrolled. The demographic data, the erythrocyte sedimentation rate (ESR), the ratio of neutrophil-to-lymphocyte (NLR), serum levels of Ca, hemoglobin (Hb), and IL-4 and IL-13 were compared between the study groups. Receiver operating characteristic (ROC) curve analysis, sensitivity, and specificity were also calculated by using SPSS 16.0 software.
Results: The mean age was 47.71 ± 10.88 and 55.25 ± 21.58 years in sarcoidosis and tuberculosis. Mean ESR in sarcoidosis patients was 21.45 ± 13.37 mm / h and 41.4 ± 17 mm / h in the TB group. The percentage of peripheral blood lymphocytes in patients with sarcoidosis and tuberculosis was 28.02 ± 12.20 and 21.41 ± 12.49, respectively, which were significantly higher among patients with sarcoidosis. NLR also 2.4 ± 1.6 and 4.4 ± 2.9 in patients with sarcoidosis and TB, respectively, which showed significant differences between the groups. Regarding the evaluation of the level of IL-4 and IL-13 in patients, it should be noted that IL-4 in patients with sarcoidosis was 90 pg / ml compared to 20 pg / ml for TB patients (P <0.001).
There is no significant difference in the level of IL-13 in the TB and the control group, which varies between 20 and 80 pg / ml (P = 0.35). However, its value was significantly higher in patients with sarcoidosis (P = 0.01) compared to the healthy control group and tuberculosis (P = 0.01). ROC curves showed that the diagnostic cutoff level of ESR, Ca, NLR, and Hb can be valuable because the area under the curve. The TLC cutpoint of 34 mm / hour to ESR had 86% sensitivity and 80% specificity to distinguish TB from the sarcoidosis.
Conclusion: The serum levels of the biomarker showed a strong background in immunology of sarcoidosis using NLR, Ca, ESR, and Hb.
he Evaluation of interleukin-4 and interleukin–13 in the Serum of Pulmonary Sarcoidosis and Tuberculosis Patients
Novel role of interleukin 13 alpha2 receptor in perineural Invasion and association with Poor Prognosis of Patients with Pancreatic Ductal Adenocarcinoma
Perineural invasion (PNI) is one of the main pathologic characteristics of pancreatic ductal adeno-carcinoma (PDAC), which is mediated by attacking cancer cells into nerve cells. Here, we identify the overexpression of interleukin-13 receptor alpha2 (IL-13Rα2) in PNI of 236 samples of PDAC by studying the expression on protein level by immunohistochemistry (IHC) and the level of RNA by in situ hybridization (ISH). We observe that ≥75% of the sample expressed IL-13Rα2 by IHC and ISH in grade 2 and 3 tumors, whereas ≥64% stage II and III tumors expressed IL-13Rα2 (≥2 +).
Interestingly, ≥36% peripancreatic nerve plexus (PL) and ≥70% nerve ends (Ne) between PNI in PDAC samples showed higher levels of IL-13Rα2 (≥2 +). IL-13Rα2 PL and Ne ve subjects survived significantly less than IL-13Rα2 ve subjects, suggesting that IL-13Rα2 may have a unique role as a biomarker of PNI-aggressiveness.
Human Interleukin 1 Receptor Accessory Protein (IL1RAP) Protein
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Interleukin 1 Receptor Accessory Protein (IL1RAP) in serum, plasma and other biological fluids.
Human Interleukin 1 Receptor Accessory Protein (IL1RAP)CLIA Kit
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Interleukin 1 Receptor Accessory Protein (IL1RAP) in serum, plasma and other biological fluids.
Human Interleukin 1 Receptor Accessory Protein (IL1RAP)CLIA Kit
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Interleukin 1 Receptor Accessory Protein (IL1RAP) in serum, plasma and other biological fluids.
Human Interleukin 1 Receptor Accessory Protein (IL1RAP)CLIA Kit
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Interleukin 1 Receptor Accessory Protein (IL1RAP) in serum, plasma and other biological fluids.
Human Interleukin 1 Receptor Accessory Protein (IL1RAP) CLIA Kit
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Interleukin 1 Receptor Accessory Protein (IL1RAP)Serum, plasma and other biological fluids
Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP)CLIA Kit
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP) in serum, plasma and other biological fluids.
Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP)CLIA Kit
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP) in serum, plasma and other biological fluids.
Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP)CLIA Kit
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP) in serum, plasma and other biological fluids.
Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP)CLIA Kit
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP) in serum, plasma and other biological fluids.
Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP) CLIA Kit
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP)Serum, plasma and other biological fluids
Human Interleukin 1 Receptor Accessory Protein (IL1RAP) CLIA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Interleukin 1 Receptor Accessory Protein (IL1RAP) in samples from serum, plasma, tissue homogenates or other biological fluids.
Human Interleukin 1 Receptor Accessory Protein (IL1RAP) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Interleukin 1 Receptor Accessory Protein (IL1RAP) in samples from serum, plasma, tissue homogenates or other biological fluids.
Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Interleukin 1 Receptor Accessory Protein ELISA Kit (IL1RAP)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 1 Receptor Accessory Protein (IL1RAP) in serum, plasma, tissue homogenates and other biological fluids.
Human Interleukin 1 Receptor Accessory Protein (IL1RAP) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 1 Receptor Accessory Protein (IL1RAP) in serum, plasma, tissue homogenates and other biological fluids.
Human Interleukin 1 Receptor Accessory Protein (IL1RAP) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 1 Receptor Accessory Protein (IL1RAP) in serum, plasma, tissue homogenates and other biological fluids.
Human Interleukin 1 Receptor Accessory Protein (IL1RAP) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 1 Receptor Accessory Protein (IL1RAP) in serum, plasma, tissue homogenates and other biological fluids.
Human Interleukin 1 Receptor Accessory Protein (IL1RAP) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Interleukin 1 Receptor Accessory Protein (IL1RAP) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.
Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP) in serum, plasma, tissue homogenates and other biological fluids.
Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP) in serum, plasma, tissue homogenates and other biological fluids.
Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP) in serum, plasma, tissue homogenates and other biological fluids.
Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP) in serum, plasma, tissue homogenates and other biological fluids.
Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Interleukin 1 Receptor Accessory Protein (IL1RAP) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.
Human Interleukin 1 Receptor Accessory Protein (IL1RAP)ELISA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL1RAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL1RAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL1RAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL1RAP in the samples is then determined by comparing the OD of the samples to the standard curve.
Human IL1RAP(Interleukin 1 Receptor Accessory Protein) ELISA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL1RAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL1RAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL1RAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL1RAP in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL1RAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL1RAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL1RAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL1RAP in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL1RAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL1RAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL1RAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL1RAP in the samples is then determined by comparing the OD of the samples to the standard curve.
Interleukin 1 Receptor Accessory Protein Like 1 (IL1RAPL1) Antibody (HRP)
Description: Quantitativesandwich ELISA kit for measuring Human Interleukin-1 receptor accessory protein (IL1RAP) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Human Interleukin-1 receptor accessory protein(IL1RAP) ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Human Interleukin-1 receptor accessory protein(IL1RAP) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativesandwich ELISA kit for measuring Mouse Interleukin-1 receptor accessory protein (IL1RAP) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Mouse Interleukin-1 receptor accessory protein(IL1RAP) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Recombinant Interleukin 18 Receptor Accessory Protein (IL18RAP)
Description: A sandwich ELISA kit for detection of Interleukin 1 Receptor Accessory Protein from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
ELISA kit for Mouse IL1RAP (Interleukin 1 Receptor Accessory Protein)
Description: A sandwich ELISA kit for detection of Interleukin 1 Receptor Accessory Protein from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Recombinant Interleukin 1 Receptor Accessory Protein Like Protein 1 (IL1RAPL1)
Description: A sandwich quantitative ELISA assay kit for detection of Human Interleukin 18 Receptor Accessory Protein (IL18RAP) in samples from tissue homogenates or other biological fluids.
Human Interleukin 18 Receptor Accessory Protein (IL18RAP) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Interleukin 18 Receptor Accessory Protein (IL18RAP) in samples from tissue homogenates or other biological fluids.
Human Interleukin 18 Receptor Accessory Protein (IL18RAP) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 18 Receptor Accessory Protein (IL18RAP) in Tissue homogenates and other biological fluids.
Human Interleukin 18 Receptor Accessory Protein (IL18RAP) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 18 Receptor Accessory Protein (IL18RAP) in Tissue homogenates and other biological fluids.
Human Interleukin 18 Receptor Accessory Protein (IL18RAP) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 18 Receptor Accessory Protein (IL18RAP) in Tissue homogenates and other biological fluids.
Human Interleukin 18 Receptor Accessory Protein (IL18RAP) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 18 Receptor Accessory Protein (IL18RAP) in Tissue homogenates and other biological fluids.
Human Interleukin 18 Receptor Accessory Protein (IL18RAP) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Interleukin 18 Receptor Accessory Protein (IL18RAP) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Human Interleukin 18 Receptor Accessory Protein (IL18RAP) ELISA Kit
Importantly, IL-13Rα2 may be a target for therapeutic intervention, which may not only prolong the survival of patients but also help reduce the pain associated with perineural invasion. Our studies reveal a novel role of IL-13Rα2 in PNI as a key factor of the severity of the disease, thus revealing selection of targeted therapy for PDAC and to facilitate the PNI-associated pain management.
