High-affinity mutant Interleukin–13 targeted CAR T cells enhance delivery of clickable biodegradable fluorescent nanoparticles to glioblastoma.
by Ronald
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Glioblastoma (GBM), the deadliest form of brain cancer, long gift for localization issues. Chimeric antigen receptor (CAR) T cell immunotherapy has emerged as a potent strategy for treating cancer. IL-13-receptor-α2 (IL13Rα2), present in more than 75% of GBMs, has been recognized as an attractive candidate for anti-glioblastoma therapy. Here, we propose a multidisciplinary approach new for brain tumor targets using a combination of fluorescent, nanoparticle therapy and cell CAR T modified by the target-quadruple-mutant of IL13 (TQM-13) proved to have a binding of high affinity for IL13Rα2-expressing glioblastoma cell toxicity off low-target.
Azide-alkyne cycloaddition conjugation of nanoparticles onto the surface of T cells is allowed smoothly, selective, and clicking on high yield of nanoparticles. Nanoparticles clicked into the T cells were maintained for at least 8 days showed that a stable relationship and a promising window of time that is suitable for in vivo delivery.
T cell clicked with doxorubicin-loaded nanoparticles showed a higher cytotoxic effect in vitro compared to bare the T cells in vitro and in vivo T cells that express TQM-13 served as a freight shipment for nanoparticles and significantly increase the amount of nanoparticles reach the brain tumor compared with nanoparticles alone. This work represents a new platform to enable the delivery of nanoparticles for therapeutic and T cells of solid tumors.
High-affinity mutant Interleukin–13 targeted CAR T cells enhance delivery of clickable biodegradable fluorescent nanoparticles to glioblastoma.
Matrix metalloproteinase- 13 , NF-kB p65 and interleukin -1β related to the severity of knee osteoarthritis.
Knee osteoarthritis (KOA) is a common disease, especially in older people. This study investigated the expression of matrix metalloproteinase-13 (MMP-13), NF-κBp65 and interleukin (IL) -lβ in synovial tissue of patients KOA and the role of MMP-13 and NF-κBp65 signaling pathway in the pathogenesis KOA.
A total of 100 patients were enrolled KOA in our hospital from December 2015 to December 2017 and classified as either mild group KOA (Outerbridge grade 1 and 2) or a group of severe KOA (Outerbridge grade 3 and 4). non-OA patients included as controls. synovial tissue of patients in both groups were collected to detect mRNA and protein expression of MMP-13, NF-κBp65 and IL-lβ. Synovial tissue sections were subjected to hematoxylin and eosin and immunohistochemistry (SP method). cartilage tissue was observed under light microscope after staining safranin O-fast green.
Analysis of quantitative reverse transcription-PCR and Western blot showed that the expression of MMP-13, NF-κBp65 and IL-lβ in mild and severe group was substantially deregulated compared with the control group (all P <0.05). A positive correlation between MMP-13 and NF-κBp65 expression in synovial tissue identified KOA (P <0.05). Immunohistochemistry revealed that the expression of MMP-13 and NF-κBp65 associated with the severity of KOA (MMP-13: heavy, 92.54%; moderate, 76.52%; control: 32.14%; and NF-κBp65: heavy, 85, 56%; moderate, 48.12%; control: 28.32%).
This evidence suggests that the severity of KOA associated with the expression of MMP-13 and NF-κBp65. NF-kB signaling pathway can be activated during the progression of OA, which could upregulate the expression of MMP-13 and IL-1β and accelerate damage to the articular cartilage.Repeated attacks muscle mitochondria exercise conditions to meet the increasing demand for energy-adaptive response associated with improved metabolic fitness.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Interleukin 12A (IL12A) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Interleukin 12A (IL12A) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Dog Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Dog Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Dog Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Dog Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Dog Interleukin 12A (IL12A) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Pig Interleukin 12A (IL12A) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Interleukin 12A (IL12A) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Goat Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Goat Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Goat Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Goat Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Goat Interleukin 12A (IL12A) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Description: This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay(ELISA). It is developed for quantitive measurement of Human IL12A in serum, plasma and other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Interleukin 12A (IL12A) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Interleukin 12A (IL12A) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Interleukin 12A (IL12A) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Interleukin 12A (IL12A) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Horse Interleukin 12A (IL12A) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Horse Interleukin 12A (IL12A) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Horse Interleukin 12A (IL12A) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Horse Interleukin 12A (IL12A) in serum, plasma and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Horse Interleukin 12A (IL12A) in samples from Serum, plasma and other biological fluids. with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Interleukin 12A (IL12A) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Interleukin 12A (IL12A) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Interleukin 12A (IL12A) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Interleukin 12A (IL12A) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Interleukin 12A (IL12A) in serum, plasma, tissue homogenates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Interleukin 12A (IL12A) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Cattle Interleukin 12A (IL12A) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Gallus Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Gallus Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Gallus Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Gallus Interleukin 12A (IL12A) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Gallus Interleukin 12A (IL12A) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Interleukin 12A (IL12A) Polyclonal Antibody (Pig), PE
Description: A sandwich quantitative ELISA assay kit for detection of Chicken Interleukin 12A (IL12A) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Chicken Interleukin 12A (IL12A) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Interleukin 12A (IL12A) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Interleukin 12A (IL12A) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
We found that type 2 cytokine interleukin-13 (IL-13) is induced in the exercising muscle, where he orchestrates metabolic reprogramming is preserved glycogen in favor fatty acid oxidation and mitochondrial respiration.
